Search results for "3d microscopy"
showing 9 items of 9 documents
Fourier-domain lightfield microscopy: a new paradigm in 3D microscopy
2020
Recently, integral (also known as lightfield or plenoptic) imaging concept has been applied successfully to microscopy. The main advantage of lightfield microscopy when compared with conventional 3D imaging techniques is that it offers the possibility of capturing the 3D information of the sample after a single shot. However, integral microscopy is now facing many challenges, like improving the resolution and depth of field of the reconstructed specimens or the development and optimization of specially-adapted reconstruction algorithms. This contribution is devoted to review a new paradigm in lightfield microscopy, namely, the Fourier-domain integral microscope (FiMic), that improves the ca…
Chapter 1 The Resolution Challenge in 3D Optical Microscopy
2009
Publisher Summary This chapter discusses the theoretical principles of 3D microscopy with the widespread realizations of 3D microscopy.Based on the paraxial diffraction equations, it has been shown that conventional microscopes, when dealing with 3D fluorescent samples, provide sets of 2D images. These images of the different transverse sections of the 3D object contain, in addition to the sharp image of the in focus section, the blurred images of the rest of the specimen. The paraxial formalism has been generalized in a very simple way to a non-paraxial context, showing that the equations that govern non-paraxial imaging are similar to those that govern paraxial imaging. The only differenc…
High-resolution deep view microscopy of cells and tissues
2020
Abstract Methods, experimental setups and perspectives of three-dimensional deep view imaging microscopy of cell or tissue samples are reported. Preliminary biophysical and clinically relevant examples are presented.
Machine Learning-Based View Synthesis in Fourier Lightfield Microscopy
2022
Current interest in Fourier lightfield microscopy is increasing, due to its ability to acquire 3D images of thick dynamic samples. This technique is based on simultaneously capturing, in a single shot, and with a monocular setup, a number of orthographic perspective views of 3D microscopic samples. An essential feature of Fourier lightfield microscopy is that the number of acquired views is low, due to the trade-off relationship existing between the number of views and their corresponding lateral resolution. Therefore, it is important to have a tool for the generation of a high number of synthesized view images, without compromising their lateral resolution. In this context we investigate h…
The Lightfield Microscope Eyepiece
2021
Lightfield microscopy has raised growing interest in the last few years. Its ability to get three-dimensional information about the sample in a single shot makes it suitable for many applications in which time resolution is fundamental. In this paper we present a novel device, which is capable of converting any conventional microscope into a lightfield microscope. Based on the Fourier integral microscope concept, we designed the lightfield microscope eyepiece. This is coupled to the eyepiece port, to let the user exploit all the host microscope’s components (objective turret, illumination systems, translation stage, etc.) and get a 3D reconstruction of the sample. After the optical design, …
Removing striping artifacts in light-sheet fluorescence microscopy: a review
2022
Abstract In recent years, light-sheet fluorescence microscopy (LSFM) has found a broad application for imaging of diverse biological samples, ranging from sub-cellular structures to whole animals, both in-vivo and ex-vivo, owing to its many advantages relative to point-scanning methods. By providing the selective illumination of sample single planes, LSFM achieves an intrinsic optical sectioning and direct 2D image acquisition, with low out-of-focus fluorescence background, sample photo-damage and photo-bleaching. On the other hand, such an illumination scheme is prone to light absorption or scattering effects, which lead to uneven illumination and striping artifacts in the images, oriented…
Capture and visualization of biological 3D samples through lightfield imaging and display techniques
2022
La microscopía óptica convencional proporciona imágenes de alta resolución de muestras microscópicas. Sin embargo, su escasa profundidad de campo y su falta de seccionado óptico hacen que sea inadecuada para la observación de especímenes tridimensionales. Para solucionar este problema, se han propuesto varias técnicas de microscopía 3D, la mayoría de ellas basadas en procedimientos de escaneo. La microscopía lightfield, en cambio, es una técnica de reciente desarrollo, que es capaz de capturar información 3D de muestras gruesas en una sola toma. Esto se consigue registrando de forma simultánea la información espacial y angular de los rayos procedentes de cada punto del objeto. Esta caracter…
Fast Axial Scanning in 3D Imaging
2016
We establish the conditions for obtaining fast axial 3D microscopy with constant magnification, invariant resolution, and symmetrical displacement range. We demonstrate that liquid lenses permit the axial scanning free of mechanical movements and vibrations. This allows speeding-up the process and avoiding distortionsin the image of dynamic specimens. Article not available.
Optical sectioning microscopy through single-shot Lightfield protocol
2020
Optical sectioning microscopy is usually performed by means of a scanning, multi-shot procedure in combination with non-uniform illumination. In this paper, we change the paradigm and report a method that is based in the light field concept, and that provides optical sectioning for 3D microscopy images after a single-shot capture. To do this we fi rst capture multiple orthographic perspectives of the sample by means of Fourier-domain integral microscopy (FiMic). The second stage of our protocol is the application of a novel refocusing algorithm that is able to produce optical sectioning in real time, and with no resolution worsening, in the case of sparse f luorescent samples.We provide the…